Cortisol Quantitative Test Reagent
Cortisol belongs to the glucocorticoid in the adrenal cortex and can be usedclinically to diagnose Cushing's syndrome and Addison's syndrome.
Features：
● Animal: Canine, Feline
● Sample type: Plasma, Serum
● Sample volume: 75μL
● Detection range：10-1000nmol/L
● Reaction time: 15 min
● 4-30°storage, 24 months shelf life
| Item | Cortisol | |||
| Specimen/Samle Vol | Serum/（EDTA-K2 Plasma） 75uL | |||
| Sample amount of mixture | 75uL | |||
| Response time | 15min | |||
| Project significance and indications | Cortisol is the glucocorticoid of the adrenocortical hormone. Clinical application: Diagnosis of Cushing's syndrome and Edison's syndrome. |
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Reference range of Cortisol in canine or feline blood: （1 ug/dL=27.64 nmol/L）
1.Detection range: 10~1000 nmol/L
2.ACTH stimulation test: intramuscular injection of ACTH 2.2 IU/kg, the maximum does not exceed 40 IU, cortisol after 2 hours. The diagnosis is as follows:
|
Pre-stimulus Cortisol value |
Cortisol value after stimulation |
Diagnostic value |
|
<55nmol/L（<2 μg/dL） |
<55nmol/L （<2 μg/dL） |
It needs to be evaluated in combination with cortisol test value after stimulation, Addison's disease. |
|
55-166nmol/L（2-6μg/dL） |
Uncertain |
|
|
55-166nmol/L（2-6μg/dL） |
55-166nmol/L |
Uncertain |
|
166-500nmol/L |
Basically normal |
|
|
500-663nmol/L |
Suspected Cushing's syndrome |
|
|
>663nmol/L |
Cushing's syndrome requires high-dose dexamethasone suppression test to distinguish between ATH or PDH. |
3.LDDST
Intravenous injection of hexadecadrol 0.01mg/kg ，and cortisol value was measured 4 hours and 8 hours later. The diagnosis was as follows:
|
type |
Cortisol value after 4 hours |
Cortisol value after 8 hours |
Diagnostic value |
|
LDDST |
- |
＜27.64nmol/L (＜1ug/dL) |
normal |
|
27.64-39nmol/L (1-1.4ug/dL) |
27.64-39nmol/L (1-1.4ug/dL) |
Suspected |
|
|
＞39nmol/L and ＞50% base value |
＞39nmol/L and ＞50% base value |
Cushing's syndrome |
|
|
＜39nmol/L or ＜50% base value |
＞39nmol/L and ＞50% base value |
PDH |
|
|
＞39nmol/L or ＞50% base value |
＞39nmol/L and ＜50% base value |
PDH |
|
|
＜39nmol/L or ＜50% base value |
＞39nmol/L and ＜50% base value |
PDH |
4. HDDST
Intravenous injection of hexadecadrol 0.1mg/kg ，and cortisol value was measured 4hours and 8 hours later. The diagnosis was as follows:
|
type |
Cortisol value after 4 hours |
Cortisol value after 8 hours |
Diagnostic value |
|
HDDST |
＜39nmol/L or ＜50% base value |
＞39nmol/L and ＞50% base value |
PDH |
|
＞39nmol/L and ＞50% base value |
＜39nmol/L or ＜50% base value |
PDH |
|
|
＜39nmol/L or ＜50% base value |
＜39nmol/L or ＜50% base value |
PDH |
|
|
＞39nmol/L and ＞50% base value |
＞39nmol/L and ＞50% base value |
More tests are needed to distinguish PDH and primary adrenal hyperactivity |
Each Laboratory should establish a reference range that is representative of the population to be evaluated. The diagnosis must be made in conjunction with other clinical symptoms and tests.
